Inhibition of chlorophyll synthesis and carotenoid accumulation by manganese and cobalt

Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6989325

Clairmont, K B; Davis, E; Hagar, W

The authors have developed methods for the separation and identification of the major pigments of the photosynthetic apparatus in plants using reversed phase microbore high performance liquid chromatography. Using these methods they have monitored the concentrations of pigments in tissue cultured tobacco callus in the absence and presence of excess manganese and cobalt. Manganese and cobalt were reported to inhibit chlorophyll synthesis in blue green algae. They have found that excess manganese blocks chlorophyll synthesis in tobacco callus also. In the manganese inhibited callus there is an increase in the concentration of protoporphyrin IX- the last common precursor to both the chlorophyll and heme synthetic pathways. They have found that cobalt also blocks chlorophyll synthesis in tissue cultured tobacco callus, but at a much lower concentration. In addition to the inhibition of chlorophyll synthesis by excess manganese and cobalt, the accumulation of carotenoids is reduced by several orders of magnitude in this tissue. The absence of chlorophyll may prevent assembly of any components of the photosynthetic apparatus in these cells.

• Research Organization: Univ. of Massachusetts, Boston
• OSTI ID: 6989325
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CAROTENOIDS
INHIBITION
CHLOROPHYLL
BIOSYNTHESIS
COBALT
BIOLOGICAL EFFECTS
MANGANESE
LIQUID COLUMN CHROMATOGRAPHY
NICOTIANA
TISSUE CULTURES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ELEMENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SYNTHESIS
TERPENES
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology