Physiological effects of ozone on tissue and homogenate systems of Nicotiana tobacum L

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1970

OSTI ID:6525252

Anderson, W C

Callus derived from Bel-W3 and Bel-B tobacco plants when exposed to ozone turned brown as a consequence of surface cell destruction. Ozone fumigations above a threshold concentration of 10 pphm for two hours caused an increase in the rate of tissue carbon dioxide (CO/sub 2/) evolution. The maximum increase in CO/sub 2/ evolution was about 65 percent for both the ozone sensitive Bel-W3 and resistant Bel-B callus. However, the ozone dosage required to attain maximum increase in CO/sub 2/ evolution was approximately two times greater for the resistant variety. Callus cultures that grew roots were observed to be more resistant to ozone. Analyses of chlorophyll and carotenoid content of foliage two days after ozone exposure showed that both pigments were destroyed in the same ratio. The use of pigment destruction as a bioassay of phytotoxicant injury is limited since the chlorophyll content per unit area varies with leaf maturity. Ozone fumigation caused the plants to increase their CO/sub 2/ evolution in the post dark cycles in a similar manner to callus. Carbon dioxide assimilation was inhibited after ozone exposure. This inhibition of CO/sub 2/ production was directly proportional to ozone dosage.

• Research Organization: California Univ., Riverside (USA)
• OSTI ID: 6525252
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CAROTENOIDS
CHALCOGENIDES
CHEMICAL REACTIONS
CHLOROPHYLL
COMPARATIVE EVALUATIONS
CORRELATIONS
DECOMPOSITION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
LEAVES
NICOTIANA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
OZONE
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
RESPIRATION
SENSITIVITY
STIMULATION
TERPENES