Manganese toxicity to chlorophyll synthesis in tobacco callus. [Nicotiana tabacum]

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:5527460

Clairmont, K B; Hagar, W G; Davis, E A

Tobacco (Nicotiana tabacum) pith explants were grown on manganese containing medium. At moderate concentration (10 millimolar), manganese selectivity inhibited chlorophyll synthesis, resulting initially in growth of white callus. Several weeks later the white callus turned brown due to the accumulation of a pigment identified as protoporphyrin IX by its elution profile using high performance liquid chromatography, by its absorption spectrum, and by its fluorescence properties. At a concentration of 100 millimolar manganese the pigment accumulated without growth of the explant.

• Research Organization: Univ. of Massachusetts, Boston
• OSTI ID: 5527460
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550500 -- Metabolism
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTRA
BIOLOGICAL ACCUMULATION
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLOROPHYLL
CHROMATOGRAPHY
DATA
ELEMENTS
EXPERIMENTAL DATA
FLUORESCENCE
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
INHIBITION
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
MANGANESE
METALS
NICOTIANA
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYTOCHROMES
PIGMENTS
PLANT GROWTH
PLANTS
PORPHYRINS
PRECURSOR
PROTEINS
PROTOPORPHYRINS
SEPARATION PROCESSES
SPECTRA
SYNTHESIS
TOXICITY
TRANSITION ELEMENTS