Inhibition of chlorophyll synthesis and carotenoid accumulation by manganese and cobalt

Journal Article · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6989325

Clairmont, K B; Davis, E; Hagar, W

The authors have developed methods for the separation and identification of the major pigments of the photosynthetic apparatus in plants using reversed phase microbore high performance liquid chromatography. Using these methods they have monitored the concentrations of pigments in tissue cultured tobacco callus in the absence and presence of excess manganese and cobalt. Manganese and cobalt were reported to inhibit chlorophyll synthesis in blue green algae. They have found that excess manganese blocks chlorophyll synthesis in tobacco callus also. In the manganese inhibited callus there is an increase in the concentration of protoporphyrin IX- the last common precursor to both the chlorophyll and heme synthetic pathways. They have found that cobalt also blocks chlorophyll synthesis in tissue cultured tobacco callus, but at a much lower concentration. In addition to the inhibition of chlorophyll synthesis by excess manganese and cobalt, the accumulation of carotenoids is reduced by several orders of magnitude in this tissue. The absence of chlorophyll may prevent assembly of any components of the photosynthetic apparatus in these cells.

• Research Organization: Univ. of Massachusetts, Boston
• OSTI ID: 6989325
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CAROTENOIDS
CHLOROPHYLL
CHROMATOGRAPHY
COBALT
ELEMENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
INHIBITION
LIQUID COLUMN CHROMATOGRAPHY
MANGANESE
METALS
NICOTIANA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SYNTHESIS
TERPENES
TISSUE CULTURES
TRANSITION ELEMENTS