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Inhibition by rhodamine 123 of protein synthesis in mitochondria of normal and cancer tissues

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
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The effect of the mitochondrial dye rhodamine 123 (Rho 123) on protein synthesis (PS) activity was investigated in mitochondria isolated from liver and from both chloroma and erythroleukemia tumors. Incorporation of labelled leucine into mitochondrial protein was used to measure the rate of PS. While PS specific activity was much higher in hematopoietic tumors mitochondria as compared to that of liver, the addition of increased concentration of Rho 123 in all tested organelles resulted in increased inhibition of PS to reach 75-82% with 10..mu..g/ml of the dye. Similar results were obtained with 10 ..mu..g/ml of chloramphenicol, the specific inhibitor of mitochondrial PS. Moreover, under the conditions of the study, the addition of Rho 123 to mitochondria did not trigger any ATPase activity, thus eliminating any competition for the energy source ATP between PS and ATPase.

Research Organization:
Univ. of Miami, FL
OSTI ID:
6986225
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:2; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ATP-ASE
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHLORAMPHENICOL
DATA
DIGESTIVE SYSTEM
DISEASES
DRUGS
DYES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GLANDS
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INFORMATION
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUCINE
LEUKEMIA
LIVER
MITOCHONDRIA
NEOPLASMS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOHYDROLASES
PROTEINS
REACTION KINETICS
REAGENTS
RHODAMINES
SYNTHESIS
TRACER TECHNIQUES
TUMOR CELLS