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T-2 mycotoxin inhibits mitochondrial protein synthesis

Technical Report ·
OSTI ID:6905739
; ;

The authors investigated the effect of T-2 toxin on rat liver mitochondrial protein synthesis. Isolated rat liver mitochondria were supplemented with an S-100 supernatant from rat liver and an external ATP-generating system. An in-vitro assay employing cycloheximide, and inhibitor of cytoplasmic protein synthesis, and chloramphenicol, and inhibitor of mitochondrial protein synthesis, to distinguish mitochondrial protein synthesis from the cytoplasmic process. Amino acid incorporation into mitochondria was dependent on the concentration of mitochondria and was inhibited by chloramphenicol. The rate of uptake of tritium leucine into mitochondrial protein was unaffected by the addition of T-2 toxin and was not a rate-limiting step in incorporation. However, 0.02 micrograms/ml of T-2 toxin decreased the rate of protein synthesis inhibition correlated with the amount of T-2 toxin taken up by the mitochondria. While T-2 toxin is known to inhibit eukaryotic protein synthesis, this is the first time T-2 was shown to inhibit mitochondrial protein synthesis.

Research Organization:
Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (USA)
OSTI ID:
6905739
Report Number(s):
AD-A-192861/3/XAB
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL WARFARE AGENTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHLORAMPHENICOL
CYTOPLASM
DIGESTIVE SYSTEM
DRUGS
FUNGI
FUSARIUM
GLANDS
HYDROGEN ISOTOPES
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LEUCINE
LIGHT NUCLEI
LIVER
MAMMALS
MATERIALS
MITOCHONDRIA
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PARASITES
PLANTS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SYNTHESIS
TOXIC MATERIALS
TOXICITY
TOXINS
TRACER TECHNIQUES
TRITIUM
UPTAKE
VERTEBRATES
WEAPONS
YEARS LIVING RADIOISOTOPES