Basis of differential cytotoxicity exhibited by rhodamine 123 for certain carcinoma cell types
The authors have previously demonstrated the inhibitory effect of rhodamine 123 (Rh123) on mitochondrial bioenergetic function and established mitochondrial F/sub 1/-F/sub 0/ ATPase as the primary target site for Rh123 toxicity. Further, they found that the amount of Rh123 taken up by isolated rat liver mitochondria is a linear function of mitochondrial membrane potential. Here they investigated the possibility that the differential cytotoxicity exhibited by Rh123 for certain carcinoma cell types might be a function of an increased mitochondrial membrane potential. Membrane potential in mitochondria isolated from cultured cells was measured by equilibrium distribution of /sup 86/Rb. Mitochondria from CX-1, a Rh123-sensitive carcinoma cell type, had a higher membrane potential (163 +/- 7mV) than did mitochondria from CV-1, a Rh123-insensitive, normal epithelial cell type (104 +/- 9mV). In addition polarographic determination of respiratory activity of mitochondria isolated from these cells showed that CX-1 mitochondria were more sensitive than CV-1 mitochondria to inhibition by Rh123. Finally, there was no evidence of differential sensitivity of F/sub 1/-F/sub 0/ ATPase activity to Rh123 in CX-1 vs. CV-1 mitochondria. These data strongly suggest that inherent differences in mitochondrial membrane potential contribute to the differential cytotoxicity exhibited by Rh123 for certain carcinoma cell types.
- Research Organization:
- Tufts Univ., Medford, MA
- OSTI ID:
- 6936982
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rhodamine-123: Radioiodination and evaluation as an agent for imaging and radiotherapy of certain tumors
The anti-cancer agent guttiferone-A permeabilizes mitochondrial membrane: Ensuing energetic and oxidative stress implications
Related Subjects
ATP-ASE
ENZYME ACTIVITY
RHODAMINES
BIOLOGICAL EFFECTS
TUMOR CELLS
CELL DIFFERENTIATION
SENSITIVITY
CARCINOMAS
CELL MEMBRANES
INHIBITION
MITOCHONDRIA
RUBIDIUM 86
TRACER TECHNIQUES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
AMINES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
DYES
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
PHOSPHOHYDROLASES
RADIOISOTOPES
REAGENTS
RUBIDIUM ISOTOPES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques