Rhodamine-123: Radioiodination and evaluation as an agent for imaging and radiotherapy of certain tumors

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6852506

Padmanabhan, S; Chen, L B; Corey, G; Garneau, J; Zubrowsky, L; Strauss, H W; Elmaleh, D R

Rhodamine-123 (Rh-123) is a cationic lypophilic fluorescent dye which localizes in the mitochondria of living cells. The compound subsequently clears through normal cells but is selectively retained in carcinoma cells. As a result, the compound exhibits anticarcinoma activity both in vitro and in vivo. This high selectivity prompted us to evaluate Rhodamine-123 as an agent for imaging and radiotherapy. Accordingly in the present study Rh-123 was labeled with iodine-125 by chloramine-T. Tissue distributions were studied for C-3Hf/SED brown mice bearing 1 cm diameter implanted SCC VII carcinoma; CDF-1 mice with MB49 bladder carcinoma, nude mice with human CX-1 colon carcinoma and brown mice with murine mammary tumor. The results indicated that (i) initially the radioactivity was taken up by the liver (3.4%), thyroid (3.8%), kidney (2.3%) and tumor (0.8%); (ii) the radioactivity from all the normal tissues cleared within 24 hours but not from the tumors (iii) in tumors the radioactivity remained unchanged. This resulted in higher tumor/blood (1.2%) and tumor/muscle (4.2%) ratios which allows detection of the tumors by gamma camera imaging techniques. In the case of nude mice with human imaging CX-1 colon carcinoma and brown mice with murine mammary tumor the tumor/muscle ratios were interestingly much higher - 8.9% and 9.3%, respectively. These results clearly show that radioiodinated Rh-123 is a potential agent for imaging and radiotherapy of certain tumors.

• Research Organization: Massachusetts General Hospital, Boston, MA
• OSTI ID: 6852506
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400700 -- Radiochemistry & Nuclear Chemistry
550604* -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLADDER
BLOOD
BODY
BODY FLUIDS
CAMERAS
CARBOXYLIC ACIDS
CARCINOMAS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORAMINES
DAYS LIVING RADIOISOTOPES
DETECTION
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DYES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GAMMA DETECTION
GASTROINTESTINAL TRACT
GLANDS
HALOGENATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMAGE PROCESSING
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLING
LARGE INTESTINE
LIVER
MAMMALS
MAMMARY GLANDS
MAN
MATERIALS
MEDICINE
MICE
MITOCHONDRIA
MUSCLES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
PRIMATES
PROCESSING
RADIATION DETECTION
RADIOACTIVITY
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
REAGENTS
RHODAMINES
RODENTS
THERAPEUTIC USES
THERAPY
THYROID
TISSUE DISTRIBUTION
URINARY TRACT
USES
VERTEBRATES