Pharmacokinetic studies of mouse monoclonal antibodies to a rat colon carcinoma: I. Comparison of biodistribution in normal rats, syngeneic tumor-bearing rats, or tumor-bearing nude mice

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:6707030

Laborda, J; Douillard, J Y; Burg, C; Lizzio, E F; Ridge, J; Levenbook, I; Hoffman, T ^[1]

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1. Food and Drug Administration, Bethesda, MD (USA)

The pharmacokinetics of two iodine-131-({sup 131}I) labeled murine anti-rat colon carcinoma monoclonal antibodies (D3 and E4) were compared in normal Sprague Dawley rats, syngeneic BDIX rats, or nude mice bearing that tumor. Results of antibody uptake after i.v. administration were analyzed in terms of accumulation and localization indices for normal tissues and tumor. Statistically significant differences between rat and mouse tissue biodistribution were found. D3, which reacts in vitro with the tumor and several normal rat tissues, cleared quickly from the blood of rats and was specifically targeted to several normal tissues, notably the lung. Virtually no targeting to the tumor was observed. Nude mice, however, showed a slower blood clearance and specific antibody targeting only in the tumor. Similar results were seen after injection of another antibody, E4, which is tumor-specific in vitro. Data suggest that studies on the xenogeneic nude mouse model may not necessarily be relevant to the choice of monoclonal antibodies for clinical diagnostic imaging or therapy.

• OSTI ID: 6707030
• Journal Information: Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 31:6; ISSN 0161-5505; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL VARIABILITY
BLOOD-PLASMA CLEARANCE
BODY
CARCINOMAS
CLEARANCE
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
EXPERIMENTAL NEOPLASMS
GASTROINTESTINAL TRACT
GENETIC VARIABILITY
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTESTINES
INTRAVENOUS INJECTION
IODINE 131
IODINE ISOTOPES
ISOTOPES
LARGE INTESTINE
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RATS
RODENTS
THERAPEUTIC USES
TISSUE DISTRIBUTION
USES
VERTEBRATES