Epidermal growth factor (EGF) sensitive phosphorylation of calmodulin (CAM) in A431 cell membrane
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6985884
A431, a transformed cell line, is known to contain a high concentration of EGF receptors (EGFR). Exogenous CAM, when combined with purified membrane from A431 is strongly phosphorylated in the presence of EGF. The EGF-dependent phosphorylation of CAM did not alter the normal profile of the A431 EGFR autophosphorylation, as demonstrated by SDS-PAGE and autoradiography. In addition to its EGF dependency, the presence of divalent cations is also critical for CAM phosphorylation. The presence of a divalent cation chelator, such as EGTA, caused a complete inhibition of CAM phosphorylation, which can be reversed with cations in the following order of effectiveness: Mg/sup + +/ > Mn/sup + +/ > Ca/sup + +/. Divalent cations also break up CAM into four co-migrating bands as indicated by Coomassie Blue stained gels and the corresponding autoradiograms. Double antibody precipitation followed by phospho-amino acid analysis revealed that the EGF-sensitive CAM phosphorylation occurs exclusively on the serine residue. Using radioimmunoassay, purified A431 membrane was shown to contain a significant amount of endogenous CAM. The implications of the EGF-sensitive CAM phosphorylation are currently under investigation.
- Research Organization:
- Los Alamos National Lab., NM
- OSTI ID:
- 6985884
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIBODIES
AUTORADIOGRAPHY
CALCIUM COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHELATING AGENTS
CHEMICAL REACTIONS
EGTA
GLYCOLS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INHIBITION
IONS
ISOTOPE APPLICATIONS
ISOTOPES
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RECEPTORS
SERINE
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
VALENCE
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIBODIES
AUTORADIOGRAPHY
CALCIUM COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHELATING AGENTS
CHEMICAL REACTIONS
EGTA
GLYCOLS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INHIBITION
IONS
ISOTOPE APPLICATIONS
ISOTOPES
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RECEPTORS
SERINE
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
VALENCE