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Ascites fluid containing monoclonal antibody (EGFR1) to the epidermal growth factor (EGF) receptor (EGFR) stimulates tyrosine phosphorylation in solubilized placental membranes

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7189296
;
Ascites fluid containing EGFR1 antibody precipitated EGFR prelabelled by phosphorylating human placental membranes with EGF and (..gamma..-/sup 32/P)ATP. The ascites fluid (at 1:100 dilution) was also found to stimulate phosphorylation of the solubilized placental membranes EGFR and the 35 kDa Ca/sup 2 +/-dependent substrate of the EGFR/kinase from placental membrane. In intact membranes, ascites fluid produced a small but noticeable stimulation of EGFR autophosphorylation. In solubilized membrane preparations, ascites fluid and EGF produced a comparable stimulation of phosphorylation both of the receptor and of pp35. The effect of EGF and ascites fluid appeared to be additive. Since EGFR1 does not inhibit the binding of EGF to the receptor, the data suggest that the antibody stimulates the receptor kinase activity by interacting with a site distinct from the EGF-binding site; the antibody effect is unmasked by receptor solubilization.
Research Organization:
Univ. of Calgary, Alberta
OSTI ID:
7189296
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIBODIES
ASCITES
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
FETAL MEMBRANES
HORMONES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
MEMBRANES
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PLACENTA
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SYMPTOMS
TRACER TECHNIQUES
TYROSINE