Epidermal growth factor (EGE) reduces the phosphorylation of a 50KD protein in A431 cells

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5463417

Selinfreund, R; Lin, P H; Wharton, W

Previous reports have shown that EGF induces the phosphorylation of the EGF receptor. In the present study the authors report the EGF sensitive reduction of the phosphorylation of a 50KD protein in solubilized A431 membranes. Membranes were prepared from A431 cell using a technique previously reported. Phosphorylation as determined by PAGE and autoradiography demonstrated an EGF sensitive phosphorylation of the EGF receptor at 170 and 150KD. A 50KD protein was phosphorylated in the absence of EGF. The presence of EGF decreased the phosphorylation of a 50KD protein. Prephosphorylation of the 50KD protein and the addition of EGF for increasing periods of time demonstrated no change in the phosphorylation level. These data suggest: (1) EGF reduces the phosphorylation of a 50KD protein. (2) The addition of EGF for increasing time periods with no change in the 50KD phosphorylation level might indicate that EGF is inhibiting a kinase rather than stimulating a phosphatase.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5463417
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
HORMONES
KINETICS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
REACTION KINETICS
RECEPTORS