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Effect of fructose on insulin action in adipose tissue of Wistar rats

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6985829
; ;

The present study was conducted to examine the effects of dietary fructose, with and without insulin stimulation, on glucose oxidation to carbon dioxide and on fatty acid synthesis in epididymal adipose tissue of rats. Two groups of male weanling Wistar rats were fed ad libitum 54% (W/W) carbohydrate diets containing 27% cornstarch plus either 27% D-fructose (FRU) or 27% D-glucose (GLU) for eleven weeks. Each diet also contained 16% fat and 20% protein. Neither body weights nor epididymal adipose tissue weights were significantly different between groups. Insulin action was assessed by incubating adipose tissue in Krebs-Ringer bicarbonate buffer (pH 7.4) containing 90 ..mu..moles (U-/sup 14/C)-D-glucose with and without insulin (1 mU/ml) for 1 hour, trapping the /sup 14/CO/sub 2/ on filter paper, and extracting the /sup 14/C-lipid with Dole's mixture. Means +/- SEM with identical superscripts are not different at the P < .05 level. These results indicate that FRU feeding stimulated glucose oxidation at a rate higher than that of GLU feeding and comparable to that stimulated by insulin. However, lipogenesis was lower in FRU fed than either in GLU fed rats or with insulin stimulation. FRU feeding does not alter the action of insulin on glucose oxidation or lipogenesis.

Research Organization:
Howard Univ., Washington, DC
OSTI ID:
6985829
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CONNECTIVE TISSUE
DIET
FRUCTOSE
GLUCOSE
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
TISSUES
TRACER TECHNIQUES
VERTEBRATES