Effects of fructose and graded levels of vanadate on insulin action and lipogenesis in fasted and refed rats
- Howard Univ., Washington, DC (United States) Food and Drug Administration, Washington, DC (United States)
This study investigated the effects of fructose and vanadate on the conversion of glucose to CO{sub 2} and to lipid in fasted and refed rats. Male Wistar rats weighing 150 g were fasted three days and refed three days a diet containing either glucose (GLU) or fructose (FRU) at 27% (w/w) with sodium orthovanadate at 0 (control), 25 (VO{sub 4}1) and 50 (VO{sub 4}2) mg/kg of diet. Glucose oxidation was measured by incubating epididymal adipocytes in buffer, pH 2.4, containing 0.5 mM D(U-{sup 14}C)glucose and entrapping the {sup 14}CO{sub 2} on hyamine hydroxide impregnated filter paper. {sup 14}C-Lipids were extracted with Dole's Mixture. FRU caused a higher rate of basal glucose oxidation to CO{sub 2} than GLU feeding. Basal glucose oxidation to CO{sub 2} was enhanced in a dose-related fashion by GLU-VO{sub 4}, but greatly inhibited by FRU-VO{sub 4} treatments. Basal and insulin-stimulated glucose conversion to lipid was inhibited by VO{sub 4} irrespective of fructose. VO{sub 4} also resulted in the inhibition of hepatic glucose 6-phosphate dehydrogenase and malic enzyme activities. These results suggest that the insulin-mimetic aspect of vanadate in stimulating glucose oxidation to CO{sub 2} is removed by fructose and that vanadate inhibits lipogenesis irrespective of fructose.
- OSTI ID:
- 5300147
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Protein quality and quantity and insulin control of mammary gland glucose utilization during lactation
High fat feeding results in a decrease in insulin responsiveness of isolated solei
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FASTING
FRUCTOSE
GLUCOSE
HEXOSES
HORMONES
INHIBITION
INSULIN
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PROTEINS
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRANSITION ELEMENTS
VANADIUM
VERTEBRATES