In vitro demonstration of anti-lipogenic activity in serum from obese rats

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5206789

Harris, R B.S.; Martin, R J

Studies with parabiosed rats provide evidence for a humoral factor, originating in obese animals, that specifically inhibits adipose lipogenesis. A bioassay was developed that allows serum from obese rats to be tested for this factor in vitro. Adipocytes are isolated from epididymal fat of 250g Sprague-Dawley rats. The cells are preincubated at 37/sup 0/C for 1 or 12 hrs, in TC199 media containing 1.1 mg/ml glucose, 0.1 M Hepes and 2% serum. Following preincubation, the cells are washed 3 times and resuspended in serum-free media. Aliquots of cells are tested for metabolic activity in a subsequent 2 hour radiolabelled incubation in serum-free media with the addition of 0.5 ..mu..Ci/ml U-/sup 14/C-glucose. Basal, insulin (100 ..mu..U/ml) and norepinephrine (0.1 ..mu..g/ml) stimulated rates of glucose oxidation and conversion to triglyceride fatty acids are measured. Using serum from ad libitum fed rats as control, preincubation with serum from obese rats (20 days at 2 x normal intake) depressed basal and insulin stimulated glucose oxidation, and basal fatty acid synthesis. Serum from obese parabiotic rats and parabiotic partners of obese rats depressed basal fatty acid synthesis. This assay allows us to test serum for anti-lipogenic activity and may be used to identify the factor responsible for this activity in obese animals.

• Research Organization: Univ. of Georgia, Athens
• OSTI ID: 5206789
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ADRENAL HORMONES
ALDEHYDES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOASSAY
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
EPIDERMIS
EPITHELIUM
ETIOLOGY
FAT CELLS
FUNCTIONS
GLUCOSE
HEXOSES
HORMONES
IN VITRO
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLIC DISEASES
METABOLISM
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NEUROREGULATORS
NORADRENALINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RATS
RODENTS
SACCHARIDES
SKIN
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES