Effects of a tumor promoter on phospholipid metabolism in HeLa cells
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:6983217
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a marked stimulation of inorganic (/sup 32/P)orthophosphate incorporation into HeLa-cell phosphatidylcholine (PC), phosphatidylethanolamine (PE), and lysophosphatidylethanolamine. The increased incorporation of inorganic (/sup 32/P)orthophosphate into PE and lysophosphatidylethanolamine in the presence of TPA was not associated with an increase in PE synthesis as detected by the incorporation of (/sup 3/H)serine or (/sup 3/H)ethanolamine. The PC-specific exchange protein from beef liver was used to insert PC labeled with (/sup 3/H)choline, inorganic (/sup 32/P)orthophosphate, or (/sup 14/C)arachidonic acid plus (/sup 3/H)palmitic acid into the outer monolayer of intact HeLa cell membranes. Radioactivity from the latter two compounds was rapidly incorporated into PE and lysophosphatidylethanolamine; the incorporation was stimulated by TPA. It was concluded that TPA stimulated the formation of PE by base exchange between ethanolamine and PC.
- OSTI ID:
- 6983217
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43:11; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Differential alterations of phospholipid metabolism in cultured cells of neural origin by phorbol esters, fatty acids, diacylglycerols and related compounds
Effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on phosphatidylethanolamine metabolism in HeLa cells
Enhanced incorporation of radioactive inorganic phosphate into phospholipids of HeLa cells by tumor promoters
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:7245127
Effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on phosphatidylethanolamine metabolism in HeLa cells
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6984946
Enhanced incorporation of radioactive inorganic phosphate into phospholipids of HeLa cells by tumor promoters
Journal Article
·
Fri Dec 31 23:00:00 EST 1982
· Carcinogenesis (N.Y.); (United States)
·
OSTI ID:5999249
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARCINOGENS
DAYS LIVING RADIOISOTOPES
ESTERS
HELA CELLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
METABOLISM
METABOLITES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROMOTERS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR PROMOTERS
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARCINOGENS
DAYS LIVING RADIOISOTOPES
ESTERS
HELA CELLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
METABOLISM
METABOLITES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROMOTERS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR PROMOTERS