Differential alterations of phospholipid metabolism in cultured cells of neural origin by phorbol esters, fatty acids, diacylglycerols and related compounds

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7245127

Cook, H W; Spence, M W

The uptake and metabolism of (/sup 3/H)methylcholine, (1,2-/sup 14/C)-ethanolamine, (1-/sup 14/C)fatty acids and (/sup 32/P) were studied in glioma (C6), neuroblastoma (N1E-115) and neuroblastoma-glioma hybrid (NG108-15) cells in culture in the presence of tetradecanoylphorbolacetate (TPA) and related analogues, fatty acids and diacylglycerol (DAG) to assess mechanisms of stimulation of phospholipid synthesis. Choline incorporation into phosphatidylcholine (PC) was stimulated 1.5-3 fold by phorbol esters and 3-10 fold by 18:1(n-9) in C6 cultures; these agents were without effect on N1E-115 and had intermediate effects on NG108-15 cells. Stimulation of (/sup 32/P) incorporation was predominantly into PC, ethanolamine incorporation into phosphatidylethanolamine (PE) was less stimulated (<1.7-fold), and 18:2(n-6) incorporation into PC or PE was not stimulated. TPA stimulated both uptake of medium (/sup 3/H)choline and its incorporation via intracellular phosphocholine into PC whereas exogenous 18:1(n-9) stimulated only utilization of intracellular P-choline in C6 cells. Choline incorporation into PC and relative stimulation by TPA or 18:1 was influenced by medium glucose and choline. Thus, metabolism of phospholipids and their precursors in neural cells can be markedly influenced by phorbol esters and fatty acids but this stimulation is dependent on cell type, growth medium, phospholipid class and nature of the stimulator.

• Research Organization: Dalhousie Univ., Halifax, Nova Scotia
• OSTI ID: 7245127
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism &amp; Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARCINOGENS
ESTERS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS