Enhanced incorporation of radioactive inorganic phosphate into phospholipids of HeLa cells by tumor promoters
Journal Article
·
· Carcinogenesis (N.Y.); (United States)
Teleocidin, a new tumor promoter, increased incorporation of radioactive inorganic phosphate (/sup 32/P/sub i/) into phospholipids in HeLa cells. This effect was detected within 1 h on incubation of the cells in medium containing teleocidin. The half-maximum effective dose of teleocidin was approximately 10 ng/ml. The main effect of teleocidin was on the incorporation of /sup 32/P/sub i/ into the phosphatidylcholine fraction, with a lesser effect on /sup 32/P/sub i/ incorporation into other phospholipid fractions. Increased incorporation of /sup 32/P/sub i/ into phospholipids was also observed on incubation of the cells with 12-O-tetradecanoylphorbol-13-acetate (TPA), dihydroteleocidin B, or lyngbyatoxin A, which are all complete tumor promoters, and also with mezerein, which is an incomplete and second stage promoter. On the other hand, at concentrations of up to 1 microgram/ml, 4-O-methyl TPA and C/sub a//sup 2/+ ionophore A23187, which are incomplete and first stage promoters, and phorbol, which has no promoting activity in skin carcinogenesis, did not cause any increased incorporation of /sup 32/P/sub i/ into phospholipid fractions of HeLa cells.
- Research Organization:
- National Cancer Center Research Institute, Tokyo, Japan
- OSTI ID:
- 5999249
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:1; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CARCINOGENS
CELL CULTURES
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ESTERS
HELA CELLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
LIPIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PATHOGENESIS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROMOTERS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TUMOR PROMOTERS
UPTAKE
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CARCINOGENS
CELL CULTURES
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ESTERS
HELA CELLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
LIPIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PATHOGENESIS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROMOTERS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TUMOR PROMOTERS
UPTAKE