Identification and regional localization of a human IMP dehydrogenase-like locus (IMPHDL1) at 16p13. 13
- Los Alamos National Lab., NM (United States)
- Adelaide Children's Hospital, North Adelaide (Australia)
- Univ. of Pittsburgh, PA (United States)
Sequence-tagged sites (STS)s are versatile chromosomal markers for a variety of genome mapping efforts. In this report, the authors describe a randomly generated STS (323F4) from human chromosome 16 genomic DNA that has 90.0% sequence identity to the type I human inosine-5[prime]-monophosphate dehydrogenase (IMPDH1) gene and 72% identity to the type II human inosine-5[prime]-monophosphate dehydrogenase (IMPDH2) gene. Additional sequencing by primer walking has provided a total of 1380 bp of the human chromosome 16 sequence. The IMPDH-like sequence 323F4 was regionally localized by PCR analysis of a panel of somatic cell hybrids containing different portions of human chromosome 16 to 16p13.3-13.12, between the breakpoints found in hybrids CY196/CY197 and CY198. This regional mapping assignment was further refined to subband 16p13.3 by high-resolution fluorescence in situ hybridization using cosmid 323F4 as a probe. The authors conclude that a third, previously undescribed IMPDH locus, termed IMPDHL1, exists at human chromosome 16p13.13. 11 refs., 2 figs.
- DOE Contract Number:
- W-7405-ENG-36; FG02-89ER60863
- OSTI ID:
- 6975250
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:3; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Assignment of the human type I IMP dehydrogenase gene (IMPDH1) to chromosome 7q31.3-q32
Chromosomal localization and structure of the human type II IMP dehydrogenase gene