Chromosomal localization and structure of the human type II IMP dehydrogenase gene
- Chicago Univ., IL (United States). Dept. of Molecular Genetics and Cell Biology
- Argonne National Lab., IL (United States)
- Colorado Univ., Denver, CO (United States). Health Sciences Center
We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.
- Research Organization:
- Argonne National Lab., IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); Department of Health and Human Services, Washington, DC (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 10148872
- Report Number(s):
- ANL/BIM/PP--77638; ON: DE94011499; CNN: Grant HG00358
- Country of Publication:
- United States
- Language:
- English
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