Chromosomal localization and structure of the human type II IMP dehydrogenase gene (IMPDH2)

Journal Article · Thu Apr 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1177· OSTI ID:6082612

Glesne, D; Huberman, E ^[1]; Collart, F ^[2]; Varkony, T; Drabkin, H ^[3]

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1. Univ. of Chicago, IL (United States) Argonne National Lab., IL (United States)
2. Argonne National Lab., IL (United States)
3. Univ. of Colorado, Denver (United States)

The authors determined the chromosomal localization and structure of the gene encoding human type II inosine 5[prime]-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, they screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.2 [yields] p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH (IMPDH2) were isolated, and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb. 14 refs., 2 figs., 1 tab.

• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6082612
• Journal Information: Genomics; (United States), Vol. 16:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 3
MAPPING
INOSINE
GENES
OXIDOREDUCTASES
CELL DIFFERENTIATION
CELL PROLIFERATION
CHROMOSOMES
ONCOGENIC TRANSFORMATIONS
YEASTS
AROMATICS
AZAARENES
CELL TRANSFORMATIONS
ENZYMES
EUMYCOTA
FUNGI
HETEROCYCLIC COMPOUNDS
HUMAN CHROMOSOMES
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PROTEINS
PURINES
RIBOSIDES
550400* - Genetics