Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer
Journal Article
·
· Molecular and Cellular Biology; (United States)
- Nagoya Univ. School of Medicine, Nagoya (Japan)
- Aichi Cancer Center Research Institute, Nagoya (Japan)
- Univ. of Helsinki (Finland)
The L-myc gene was first isolated from a human small-cell lung cancer (SCLC) cell line on the basis of its amplification and sequence similarity to c-myc and N-myc. A new mechanism of L-myc activation which results from the production of rlf-L-myc fusion protein was recently reported. On the basis of our earlier observation of a rearrangement involving amplified L-myc in an SCLC cell line, ACC-LC-49, we decided to investigate this rearrangement in detail along with the structure of L-myc amplification units in five additional SCLC cell lines. We report the identification of a novel genomic region, termed jal, which is distinct from rlf and is juxtaposed to and amplified with L-myc during the process of DNA amplification of the region encompassing L-myc. Long-range analysis using pulse-field gel electrophoresis revealed that the amplified L-myc locus is involved in highly complex intrachromosomal rearrangements with jal and/or rlf. Our results also suggest that the simultaneous presence of rearrangements both in rlf intron 1 and in regions immediately upstream of L-myc may be necessary for the expression of rlf-L-myc chimeric transcripts. 26 refs., 6 figs., 1 tab.
- OSTI ID:
- 6974418
- Journal Information:
- Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 12:4; ISSN 0270-7306; ISSN MCEBD4
- Country of Publication:
- United States
- Language:
- English
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