Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Cancer Research; (USA)

OSTI ID:6694070

Sturm, S A; Strauss, P G; Adolph, S; Hameister, H; Erfle, V ^[1]

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1. GSF-Abteilung fuer Molekulare Zellpathologie, Neuherberg (Germany, F.R.)

Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosome 15, was amplified concomitantly. Multiple copies of both loci were localized on double minutes. Three of the tumors with c-myc amplification also showed rearrangements of the c-myc gene region. One of these rearrangements included the 5' and 3'-flanking sequences and the noncoding part of the third exon. Repetitive sequences were found in the 5' region of the c-myc gene, and the 3' flanking region was substituted by sequences normally present in a more distant part of chromosome 15. Increased levels of c-myc transcripts of apparently normal size were found in tumors carrying amplified c-myc sequences. Abnormally high expression of c-myc in some tumors was correlated with an early stage of osteogenic differentiation, suggesting the involvement of the c-myc gene in the control of the osteogenic differentiation of transformed cells.

• OSTI ID: 6694070
• Journal Information: Cancer Research; (USA), Journal Name: Cancer Research; (USA) Vol. 50:13; ISSN 0008-5472; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL FUNCTIONS
CHROMOSOMES
DISEASES
EXPERIMENTAL NEOPLASMS
FUNCTIONS
GENE AMPLIFICATION
GENE RECOMBINATION
GENE RECOMBINATION PROTEINS
GENE REGULATION
GENES
GLYCOPROTEINS
KARYOTYPE
MAMMALS
MICE
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
OSTEOSARCOMAS
PROTEINS
RADIOINDUCTION
RNA
RODENTS
SARCOMAS
SKELETAL DISEASES
TRANSCRIPTION
VERTEBRATES