Induction of transcription from the long terminal repeat of Moloney murine sarcoma provirus by UV-irradiation, x-irradiation, and phorbol ester

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.1.36· OSTI ID:6974095

Lin, C S; Goldthwait, D A; Samols, D ^[1]

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1. Case Western Reserve Univ., Cleveland, OH (USA)

The long terminal repeat (LTR) of Moloney murine sarcoma virus (Mo-MuSV) was used as a model system to study the stress response of mammalian cells to physical carcinogens. The chloramphenicol acetyltransferase (CAT) gene was inserted between two Mo-MuSV LTRs, and the LTR-CAT-LTR construct was used for virus production and was integrated into the genome of NIH 3T3 cells in the proviral form. This construct was used to assure that the integrated CAT gene was driven by the promoter of the LTR. Expression of the CAT gene was stimulated 4-fold by UV irradiation, and the peak of activity was observed at 18 hr. In contrast, stimulation of the CAT expression after x-irradiation was 2-fold and occurred at 6 hr. Phorbol myristate acetate also stimulated CAT activity 4-fold with a peak at 6 hr. Down-regulation of protein kinase C blocked totally the response to x-irradiation but only partially the response to UV. The protein kinase inhibitor H7 blocked the response to treatment by UV, x-ray, and phorbol ester.

• DOE Contract Number: FG02-87ER60587
• OSTI ID: 6974095
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:1; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL STRESS
CARCINOGENS
CELL CONSTITUENTS
CHLORAMPHENICOL
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ENZYME INDUCTION
ENZYMES
ESTERS
EXTREME ULTRAVIOLET RADIATION
GENE REGULATION
IONIZING RADIATIONS
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHORBOL ESTERS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
SARCOMAS
TRANSCRIPTION
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES
X RADIATION