Characterization of serine kinase activity associated with Moloney murine sarcoma virus V-mos gene products

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6106736

Maxwell, S A

Various Moloney murine sarcoma virus (Mo-MuSV) isolates contain a cellular sequence, termed mos, which is responsible for the transforming ability of Mo-MuSV. A serine kinase activity has been found to be associated with mos gene product of several isolates of Mo-MuSV. A mutants of Mo-MuSV strain 124 (designated MuSV ts 110) is temperature-sensitive (ts) for transformation and encodes two proteins, P85/sup gag-mos/ and P58/sup gag/, at the permissive temperature (28/sup 0/C). At the nonpermissive temperature (39/sup 0/C), only P58/sup gag/ is found in MuSV ts 110-infected NRK cells (6m2 cells). Both P85/sup gag-mos/ and P58/sup gag/ were phosphorylated when anti-gag immune complexes containing these proteins were incubated at 22/sup 0/C with (lambda-/sup 32/P)-ATP and MnCl/sub 2/. The kinase detected in anti-gag complexes from 6m2 cells at permissive temperature was associated with P85/sup gag-mos/ since immune complexes from 39/sup 0/C 6m2 cells, which lack P85/sup gag-mos/, produced no phosphorylated P58/sup gag/ molecules. Using the optimal conditions developed for the P85/sup gag-mos/ kinase, Mo-MuSV-encoded p37/sup mos/ was also found to be associated with a serine kinase activity. Antibodies directed against the C-terminus of v-mos were found to inhibit the in vitro phosphorylation of p37/sup mos/, suggesting that the extreme C-terminal sequence of v-mos may be important for an intrinsic kinase activity. This inhibitory action by antibodies to the C-terminus of p37/sup mos/, when considered with all the other data reported here, provides convincing evidence that the v-mos gene encodes a serine protein kinase activity.

• Research Organization: Texas Univ., Houston (USA). Graduate School of Biomedical Sciences
• OSTI ID: 6106736
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
HALIDES
HALOGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MANGANESE CHLORIDES
MANGANESE COMPOUNDS
MANGANESE HALIDES
MICROORGANISMS
MUTANTS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RADIOISOTOPES
TEMPERATURE EFFECTS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
VIRUSES