Protein kinase C mediates x-ray inducibility of nuclear signal transducers EGR1 and JUN

Journal Article · Fri Mar 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.6.2156· OSTI ID:5016030

Hallahan, D E; Sukhatme, Vikas P; Virudachalam, S; Weichselbaum, R R ^[1]; Sherman, M L; Kufe, D ^[2]

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1. Univ. of Chicago, IL (United States)
2. Harvard Medical School, Boston, MA (United States)

The cellular response to ionizing radiation includes growth arrest and DNA repair followed by proliferation. Induction of immediate early response genes may participate in signal transduction preceding these phenotypic responses. The authors analyzed mRNA expression for different classes of immediate early genes (JUN, EGR1, and FOS) after cellular x-irradiation. Increased expression of the EGR1 and JUN genes was observed within 0.5-3 hr following x-ray exposure. Preincubation with cycloheximide was associated with superinduction of JUN and EGR1 in x-irradiated cells. Inhibition of protein kinase C activity by prolonged stimulation with phorbol 12-myristate 13-acetate or the protein kinase inhibitor H7 prior to irradiation attenuated the increase in EFR1 and JUN transcripts. FOS expression was not coregulated with that of EGR1 following x-irradiation, suggesting a distinct regulatory pathway of this gene as compared with its regulation following serum and phorbol ester. These data implicate the EFR1 and JUN proteins as signal transducers during the cellular response to radiation injury and suggest that this effect is mediated in part by a protein kinase C-dependent pathway.

• OSTI ID: 5016030
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:6; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL PROLIFERATION
CHEMISTRY
DNA HYBRIDIZATION
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
EPITHELIUM
GENE REGULATION
GENES
HYBRIDIZATION
IONIZING RADIATIONS
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RNA
TISSUES
TRANSFERASES
TUMOR CELLS
X RADIATION