Genomic organization and chromosomal localization of the T-cell antigen 4-1BB

Journal Article · Tue Mar 01 04:00:00 EST 1994 · Journal of Immunology; (United States)

OSTI ID:6965066

Kwon, B S; Kim, K K; Pickard, R T ^[1]; Kozak, C A ^[2]

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1. Indiana Univ. School of Medicine, Indianapolis, IN (United States)
2. National Institute of Health, Bethesda, MD (United States)

4-1BB is an inducible T cell surface receptor which belongs to the nerve growth factor receptor superfamily, a group of cysteine-rich cell-surface proteins. 4-1BB is a 30-kDa glycoprotein and exists as both a monomer and 55-kDa dimer on the T cell surface. Cross-linking 4-1BB with monoclonal antibody resulted in the 2- to 10-fold enhancement of T cell proliferation. The authors have isolated and characterized 4-1BB genomic clones and have found that the 4-1BB gene contains two different 5[prime] untranslated regions, which are used alternately to form the 4-1BB mRNA. The two 5[prime] UTRs were encoded in the same chromosome and were separated from one another by an intron of [approximately]2.5 kb. The entire gene spans approximately 13 kb of mouse chromosome 4. 4-1BB gene consists of 10 exons and 9 introns, in which there are two exons for 5[prime] untranslated regions and 8 exons for coding region. Most of the putative functional domains were encoded by separate exons. 4-1BB extracellular domain contains four potential C6 (CXn C XX C XX CXn C Xn C) motifs, of which the first motif is partial and the third is distinct from those of nerve growth factor receptor or TNF receptor 1. A comparison of exon-intron organization among the genes of the nerve growth factor receptor family indicated that most C6 motif is interrupted by an intron. 33 refs., 5 figs., 1 tab.

• OSTI ID: 6965066
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 152:5; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GROWTH FACTORS
HYBRIDIZATION
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS