Structure, organization, and transcription units of the human {alpha}-platelet-derived growth factor receptor gene, PDGFRA
- Tohoku Univ. School of Medicine, Sendai (Japan)
Isolation and characterization of genomic clones encoding human {alpha}-platelet derived growth factor receptor (HGAM-approved symbol PDGFRA) revealed that the gene spans approximately 65 kb and contains 23 exons. The 5{prime}-untranslated region of the mRNA is encoded by exon 1, and a large intron of 23 kb separates exon 2 encoding the translation initiator codon AUG and the signal sequence. The locations of exon/intron boundaries in the extracellular immunoglobulin-like domains, the transmembrane domain, the two cytoplasmic tyrosine kinase domains, and the kinase insertion domain are very similar to those in c-kit and macrophage colony stimulating factor-1 receptor genes. The transcription start site was mapped to a position 393 bp upstream of the AUG translation initiator codon by Si mapping and primer extension analysis. The 5{prime}-flanking region of the gene lacks a typical TATA box but contains a typical CCAAT box and GATA motifs. This region also contains potential sites for AP-1, AP-2, Oct-1, Oct-2, and Sp1. The 5{prime}-flanking region of the gene was fused to the luciferase reporter gene, and transcription units of the gene were determined. 49 refs., 6 figs., 1 tab.
- OSTI ID:
- 446965
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 30; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
ANIMAL CELLS
BLOOD PLATELETS
CELL DIFFERENTIATION
CELL PROLIFERATION
CODONS
DNA-CLONING
EXONS
GENETIC MAPPING
GROWTH FACTORS
HUMAN CHROMOSOMES
IMMUNOGLOBULINS
INTRONS
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RECEPTORS
SPLICING
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION
TYROSINE