Structure of the human lysyl oxidase gene

Journal Article · Wed Sep 01 04:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1369· OSTI ID:6974817

Haemaelaeinen, E R; Kemppainen, R; Pihlajaniemi, T; Kivirikko, K I ^[1]

---

1. Univ. of Oulu (Finland)

Lysyl oxidase (EC 1.4.3.13), an extracellular copper enzyme, initiates the crosslinking of collagens and elastin by catalyzing oxidative deamination of the [epsilon]-amino group in certain lysine and hydroxylysine residues. The authors report here that the human lysyl oxidase gene is about 15 kb in size and consists of seven exons. Transcription is initiated at one major site and four minor sites, and the first exon consists of 273 bp of untranslated sequences (calculated to the major site) and 631 bp of translated sequences, which accounts for about half of all the translated sequences of the gene. The seventh exon, on the other hand, codes for only the last codon of amino acid 416 and for amino acid 417, which are followed by the translation termination codon and the 3[prime] untranslated sequences. Exons 2-6 vary in size from 96to157 bp, and the introns from 331 bp to about 3.5 kb. The 5[prime] flanking region contains a TATA-like sequence at -30 relative to the major transcription initiation site and a CCAAT motif at -109. The 5[prime] flanking region and the downstream sequences present in the first exon and first intron contain altogether five possible binding sequences for Sp1, six for AP-2, one for AP-1, three of PEA3, three for MEP-1, and three CCCTCCC motifs, all of which may be involved in the regulation of the expression of the gene. 25 refs., 4 figs., 1 tab.

• OSTI ID: 6974817
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:3; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINE OXIDASES
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DEAMINATION
DNA SEQUENCING
ENZYMES
GENES
LYSINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS