Structure and chromosomal localization of the human salivary mucin gene, MUC7
- State Univ. of New York, Buffalo, NY (United States); and others
We have isolated and characterized several MUC7 genomic clones encoding the human low-molecular-weight salivary mucin, MG2. The MUC7 gene spans {approximately}10.0 kb and comprises of three exons and two introns. Intron 1 is {approximately}1.7 kb long and is located in the 5{prime}-untranslated region of the corresponding MUC7 cDNA. Intron 2 spans {approximately}6.0 kb and is located close to the boundary of the putative leader peptide and secreted protein. The entire region encoding the secreted peptide is located on exon 3, spanning {approximately}2.2 kb. The nucleotide sequence of sections of the MUC7 gene, including 1500 bp of the 5{prime}-flanking region, was determined and analyzed for motifs identical or homologous to other known response elements. A modified RACE procedure was used to determine the 5{prime}-end of the MUC7 mRNA. PCR, the human-hamster somatic cell hybrid panel PCRable DNAs kit, and an in situ hybridization analysis on the complete metaphase chromosome spreads were used for the chromosomal localization of the MUC7 gene. It was mapped to chromosome 4q13-q21. 30 refs., 3 figs.
- OSTI ID:
- 508326
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 31; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENETIC MAPPING
GLYCOPROTEINS
HUMAN CHROMOSOMES
HYBRIDIZATION
MESSENGER-RNA
NUCLEOTIDES
PEPTIDES
POLYMERASE CHAIN REACTION
SALIVARY GLANDS
SIZE
SOMATIC CELLS
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION
TRANSCRIPTION