Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose: Ceramide galactosyltransferase gene
- Univ. of North Carolina, Chapel Hill, NC (United States); and others
UDP-galactose:ceramide galactosyltransferase (CGT) (EC 2.11.62) catalyzes the final step in the synthesis of galactocerebroside, a glycosphingolipid characteristically abundant in myelin. In this report, we describe the isolation of genomic clones spanning the mouse CGT gene. The mouse CGT gene consists of six exons that span a minimum of 70 kb of DNA and that encode a 541 amino acid translation product with extensive sequence similarity to the rat CGT enzyme and to UDP-glucuronosyltransferases (UGT). The 5{prime}-untranslated region of the mouse CGT gene is encoded by a separate exon located approximately 25 kb upstream of the first protein-encoding exon. Furthermore, the genomic organization of the five encoding region exons of the mouse CGT gene resembles that of the human UGT1 and rat UGT2B1 genes. Finally, analysis of somatic cell hybrids by PCR and fluorescence in situ hybridization to metaphase chromosomes has localized the mouse CGT gene to chromosome 3, bands E3-F1. 26 refs., 5 figs., 1 tab.
- OSTI ID:
- 466037
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactosyltransferase (cerebroside synthetase)
Genomic organization and localization of the gene encoding human preprogalanin
Related Subjects
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
BIOSYNTHESIS
CEREBROSIDES
CHROMOSOMES
DNA SEQUENCING
DNA-CLONING
E CODES
EXONS
FLUORESCENCE
GENETIC MAPPING
HEXOSYL TRANSFERASES
HYBRIDIZATION
IN-SITU HYBRIDIZATION
MICE
MUTANTS
MYELIN
NUCLEOTIDES
POLYMERASE CHAIN REACTION
SOMATIC CELLS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION