Interactions of radiation and 5-fluorouracil, cyclophosphamide or methotrexate in intestinal crypt cells
Journal Article
·
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
The interactions of radiation and 5-fluorouracil (5-FU), cyclophosphamide (CTX), or methotrexate (MTX) in mouse jejunal crypt cells were studied using the microcolony survival assay. 5-FU given from 48 hr before to 24 hr after irradiation resulted in an almost constant, increased cell kill except at injection 6 hr after irradiation, which resulted in a more pronounced effect. CTX enhanced the radiation effect only when given simultaneously with or up to 3 hr after irradiation. The effect of MTX, extremely dependent on the sequence and interval between drug administration and irradiation, was most prominent when administered 1 hr before irradiation. At this drug-radiation interval, the D/sub 0/ surprisingly increased by a factor of 2.4, whereas MTX 15 min before irradiation displaced the survival curve to the left without changing the D/sub 0/. The influence of MTX on the radiation response disappeared when the drug was given either 96 hr before or 3 hr after irradiation.
- Research Organization:
- Inst. of Cancer Research, Aarhus, Denmark
- OSTI ID:
- 6964094
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 12:1; ISSN IOBPD
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Related Subjects
550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
CELL KILLING
CRYPT CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
FLUOROURACILS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSIVE DRUGS
IONIZING RADIATIONS
MAMMALS
METHOTREXATE
MICE
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
URACILS
VERTEBRATES
X RADIATION
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
CELL KILLING
CRYPT CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
FLUOROURACILS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSIVE DRUGS
IONIZING RADIATIONS
MAMMALS
METHOTREXATE
MICE
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
URACILS
VERTEBRATES
X RADIATION