Isolation and characterization of metallothioneins in calves ingesting Zn toxic diets

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6962415

Graham, T W; Clegg, M S; Lonnerdal, B; Thurmond, M C; Keen, C L

The authors have recently described an outbreak of Zn toxicosis in 95 Holstein bull calves which occurred as a result of an accidental over-supplementation of the diet with Zn. Signs of Zn toxicosis including anorexia, polydipsia, polyphagia, polyurea and diarrhea, began to appear 23 days after initiation of the diet. Liver, kidney, muscle and brain were collected from animals that died or were euthanized. Multielement analysis indicated that liver had the highest concentration of Zn (362 ..mu..g Zn/g wet wt.) followed by kidney (233 ..mu..g Zn/g wet wt.), muscle (22 ..mu..g Zn/g wet wt.) and brain (10 ..mu..g Zn/g wet wt.). To examine the toxic effects of Zn at the molecular level, liver was fractionated by conventional molecular sieve (Sephadex G75) and anion exchange chromatography (DEAE Sephadex A-25). In addition, Fast Protein Liquid Chromatography (FPLC) was used to verify the results obtained by conventional methods. The principle advantages of FPLC are that the molecular sieve (Superose 12) and anion (Mono Q) steps are performed in 1 h as opposed to several hours, thus substantially minimizing artifacts resulting from oxidative degradation of labile proteins. Zn was primarily associated with two peaks, designated metallothionein (MT) I and MT II, in a ratio of 10 to 1. The results show that one effect of Zn toxicity in calves can be a preferential induction of MT I.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6962415
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CALVES
DISEASES
METALLOTHIONEIN
CHEMICAL COMPOSITION
ZINC
TISSUE DISTRIBUTION
TOXICITY
BRAIN
ION EXCHANGE CHROMATOGRAPHY
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MULTIVARIATE ANALYSIS
MUSCLES
SYMPTOMS
ANIMALS
BODY
CATTLE
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
DOMESTIC ANIMALS
ELEMENTS
GLANDS
MAMMALS
MATHEMATICS
METALLOPROTEINS
METALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RUMINANTS
SEPARATION PROCESSES
STATISTICS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550900 - Pathology