Influence of dietary fat and selenium fed during initiation or promotion on the development of preneoplastic lesions in rat liver

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6962337

Baldwin, S; Parker, R S

Aflatoxin B/sub 1/ (AFB1)-induced ..gamma..-glutamyl transpeptidase (GGT)-positive foci in rat liver were assessed in animals fed different levels of fat and selenium (Se) during either initiation (IN) or promotion (PR). Male Sprague Dawley rats (50g) were divided into 12 groups. One of six modified AIN-76 experimental diets were fed to groups 1-6 during weeks 1-4.5 (IN) and to groups 7-12 during weeks 4.5-15 (PR). During weeks 3-4, 13 rats/group received 10 daily doses of AFB1 (.4 mg/kg bwt/dose, i.g.). Two levels of corn oil (2% and 20%) were fed, each containing 3 levels of Se: < 0.02; 0.15; 2.5 (IN) or 1.9 (PR) ppm. When not fed the experimental diets rats were fed a standard AIN-76 diet. In groups 1-6, 0.03% phenobarbital was added to the standard diet. At week 15 rats were sacrificed. Compared to all low-fat groups, the high-fat diets with either < 0.02 or 0.15 ppm Se fed during IN resulted in a marked increase in mean diameter of GGT-positive foci and % liver section occupied by foci. In rats fed high-fat 2.5 ppm Se, preneoplastic development was decreased below all low-fat groups. During PR, Se status but not dietary fat level influenced foci formation. Rats fed < 0.02 ppm Se had greater mean diameter of foci and % section occupied by foci than either 0.15 or 1.9 ppm Se. Thus, an interaction was observed between dietary fat and selenium during IN, but not during PR.

• Research Organization: Cornell Univ., Ithaca, NY
• OSTI ID: 6962337
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550900 -- Pathology
560300* -- Chemicals Metabolism &amp; Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANESTHETICS
ANIMALS
ANTICONVULSANTS
ANTIGENS
AZINES
BARBITURATES
BODY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIET
DIGESTIVE SYSTEM
DISEASES
DRUGS
ELEMENTS
FATS
GLANDS
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
LIVER
MAMMALS
MATERIALS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHENOBARBITAL
PYRIMIDINES
RATS
RODENTS
SELENIUM
SEMIMETALS
TOXIC MATERIALS
TOXICITY
TOXINS
VERTEBRATES