Receptor for acidic fibroblast growth factor is related to the tyrosine kinase encoded by the fms-like gene (FLG)

Journal Article · Tue Oct 31 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.22.8722· OSTI ID:6957923

Ruta, M; Epstein, J; Neiger, N ^[1]; Burgess, W ^[2]; Givol, D; Kaplow, J; Grumley, G; Dionne, C; Jaye, M; Schlessinger, J ^[3]

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1. Food and Drug Administration, Bethesda, MD (USA)
2. American Red Cross, Rockville, MD (USA)
3. Rorer Biotechnology, Inc., King of Prussia, PA (USA)

The authors have previously isolated a human gene from an endothelial cell cDNA library encoding a putative tyrosine kinase; they have designated this gene the fms-like gene (FLG). To analyze the gene products(s) of FLG, the authors have generated rabbit polyclonal antibodies directed against a synthetic peptide from FLG and used it to immunoprecipitate biosynthetically labeled FLG protein from a variety of human cell lines. These antibodies specifically recognized glycoprotein(s) of 100, 120, and 135 kDa with protein cores of 90 and 110 kDa. Acidic fibroblast growth factor (aFGF) stimulated tyrosine kinase activity of FLG in vitro and in living cells, suggesting that FLG encodes the membrane receptor for aFGF. Further supporting evidence came from cross-linking agent disuccinimidyl suberate and {sup 125}I-labeled aFGF as a specific probe. The cross-linked {sup 125}I-labeled aFGF-AFGF receptor complex was specifically immunoprecipitated with FLG antipeptide antibodies. It appears, therefore, that the receptor(s) for aFGF is related to the FLG gene product.

• OSTI ID: 6957923
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:22; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
GENES
GROWTH FACTORS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MESSENGER-RNA
MITOGENS
MOLECULAR STRUCTURE
MYOSARCOMAS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RHABDOMYOSARCOMAS
RNA
SARCOMAS
TRANSFERASES
TUMOR CELLS
TYROSINE