Related fibroblast growth factor receptor genes exist in the human genome

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.20.8180· OSTI ID:5803629

Houssaint, E; Champion-Arnaud, P C; Gesnel, M C; Breathnach, R ^[1]; Blanquet, P R; Torriglia, A; Courtois, Y ^[2]

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1. Univ. de Nantes, Cedex (France)
2. Institut National de la Sante, Paris (France)

The authors have isolated, from a human tumor cDNA library, a gene encoding a putative receptor-like protein-tyrosine kinase that they call TK14. The amino acid sequence of the TK14 protein is closely related to the available partial sequence of the mouse protein bek, and more distantly related to the sequences of a chicken basic fibroblast growth factor receptor (73{percent} sequence homology) and the apparent human equivalent of this receptor, the FLG protein (encoded by the fms-like tyrosine kinase gene). Overexpression of the TK14 protein by transfection of COS-1 cells with the corresponding cDNA in a simian virus 40-based expression vector leads to the appearance of new cell-surface binding sites for both acidic and basic fibroblast growth factors. This has been demonstrated by specific binding assays and chemical cross-linking experiments using {sup 125}I-labeled growth factors. It appears, therefore, that the human genome contains at least two distinct genes, for TK14 and FLG, that code for related fibroblast growth factor receptors.

• OSTI ID: 5803629
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:20; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
CELL PROLIFERATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA SEQUENCING
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FIBROBLASTS
GENES
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES