Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3

Journal Article · Fri Feb 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.4.1095· OSTI ID:6203613

Keegan, K; Hayman, M J ^[1]; Johnson, D E; Williams, L T ^[2]

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1. State Univ. of New York, Stony Brook (United States)
2. Univ. of California, San Francisco (United States)

The fibroblast growth factors are a family of polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing. Fibroblast growth factor receptors (FGFRs) have previously been identified in chicken, mouse, and human and have been shown to contain an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. The authors have isolated a human cDNA for another tyrosine kinase receptor that is highly homologous to the previously described FGFR. Expression of this receptor cDNA in COS cells directs the expression of a 125-kDa glycoprotein. They demonstrate that this cDNA encodes a biologically active receptor by showing that human acidic and basic fibroblast growth factors activate this receptor as measured by {sup 45}Ca{sup 2+} efflux assays. These data establish the existence of an additional member of the FGFR family that they have named FGFR-3.

• OSTI ID: 6203613
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:4; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
AMINO ACID SEQUENCE
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACIDS
CHICKENS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DRUGS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FIBROBLASTS
FOWL
GENES
GERM CELLS
GROWTH FACTORS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METHIONINE
MICE
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OOCYTES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TRANSFERASES
TYROSINE
VERTEBRATES