Induction of micronuclei by bleomycin in G[sub 0] human lymphocytes: II. Potentiation by radioprotectors

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850210207· OSTI ID:6954760

Hoffmann, G R; Colyer, S P; Littlefield, L G ^[1]

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1. Oak Ridge Associated Universities, TN (United States)

Dimethylsulfoxide (DMSO) and WR-1065 are radioprotectors, in that they reduce the effectiveness with which ionizing radiation causes genetic damage. Unlike their protective effects with radiation, these agents potentiate the induction of micronuclei by bleomycin in the cytokinesis-block assay in G[sub 0] human lymphocytes. High concentrations of DMSO (1 M) are required to cause potentiation. In contrast, WR-1065 causes dose-dependent potentiation at relatively low concentrations (1.25 to 10 mM). Cytogenetic analysis supports higher levels of genetic damage induced by the combination of blemycin with DMSO or WR-1065 than by bleomycin alone. Possible mechanisms of potentiation are proposed. 38 refs., 6 tabs.

• DOE Contract Number: AC05-76OR00033
• OSTI ID: 6954760
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 21:2; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BLEOMYCIN
CHROMOSOMAL ABERRATIONS
DMSO
DOSE-RESPONSE RELATIONSHIPS
DRUGS
INDUCTION
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SULFOXIDES
THIOLS
TOXICITY