Induction of micronuclei by bleomycin in G[sub 0] human lymphocytes: 1. Dose-response and distribution

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850210206· OSTI ID:7225039

Hoffmann, G R; Colyer, S P; Littlefield, L G ^[1]

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1. Oak Ridge Associated Universities, TN (United States)

The cytokinesis-block, micronucleus assay was used to investigate the induction of chromosomal damage by bleomycin in G[sub 0] human lymphocytes. A dose-dependent increase in the frequency of micronuclei was observed in binucleate cells, and the frequency approached 0.5 micronuclei per cell at the highest dosage tested. The distribution of micronuclei among cells was overdispersed, rather than fitting a Poisson distribution. Even at the highest dosage, more than two-thirds of the cells did not contain micronuclei, while some cells were highly damaged, containing more than 4 micronuclei per cell. 32 refs., 4 tabs.

• OSTI ID: 7225039
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 21:2; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BLEOMYCIN
CHROMOSOMAL ABERRATIONS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
INDUCTION
MUTATIONS
TOXICITY