An immunochemical approach to the study of DNA damage and repair

Technical Report · Fri May 01 04:00:00 EDT 1992

DOI:https://doi.org/10.2172/6952928· OSTI ID:6952928

Wallace, S S ^[1]; Erlanger, B F ^[2]

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1. Vermont Univ., Burlington, VT (United States). Dept. of Microbiology and Molecular Genetics
2. Columbia Univ., New York, NY (United States). Dept. of Microbiology

The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We have been studying damages that are stable radiolysis products found in X-irradiated DNA and thus of potential biological consequence. Our aim is to build an in vitro and in vivo data base on the interactions between model DNA lesions and such cellular enzymes as DNA polymerases and repair endonucleases. Initial studies have focused on pyrimidine ring saturation products (thymine glycol.and dihydrothymine), products resulting from ring fragmentation or base loss (urea, {Beta}-ureidoisobutyric acid, abasic sites), 7-hydro-8-oxopurines, and more recently, cytosine radiolysis products. These modified bases serve as useful models for examining the potential lethal and/or mutagenic (carcinogenic) effects of the products of DNA radiolysis.

• Research Organization: Vermont Univ., Burlington, VT (United States). Dept. of Microbiology and Molecular Genetics
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-88ER60742
• OSTI ID: 6952928
• Report Number(s): DOE/ER/60742-5; ON: DE93003122
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOASSAY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL PREPARATION
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA REPAIR
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
GENE MUTATIONS
GLOBULINS
HETEROCYCLIC COMPOUNDS
IMMUNOASSAY
IMMUNOGLOBULINS
IONIZING RADIATIONS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROGRESS REPORT
PROTEINS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOLYSIS
REPAIR
RIBOSIDES
SPECTROSCOPY
SYNTHESIS
X RADIATION