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U.S. Department of Energy
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An immunochemical approach to the study of DNA damage and repair: Technical progress report for period August 1, 1987--July 31, 1988

Technical Report ·
OSTI ID:6869127
The overall objective for this project is to develop immunochemical methods to quantitate unique DNA base damages in x-irradiated DNA. Our approach has been to chemically synthesize modified nucleotides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We are studying damages that have been shown to be stable radiolysis products found in x-irradiated DNA and thus products that have potential biological consequence. Several thymine ring saturation or fragmentation products have been chosen as models for pyrimidine radiolysis products. For the present project period, we planned to use the characterized antibodies to quantitate DNA damage x-irradiated in vitro, to immunochemically characterize the antibody to dihydrothymine, and to develop new mono and polyclonal antibodies to model modified DNA bases. 41 refs.
Research Organization:
Vermont Univ., Burlington (USA)
DOE Contract Number:
FG02-88ER60742
OSTI ID:
6869127
Report Number(s):
DOE/ER/60742-1; ON: DE89004123
Country of Publication:
United States
Language:
English