An immunochemical approach to the study of DNA damage and repair: Technical progress report, August 1, 1986 to July 31, 1987

Technical Report · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6122666

Immunochemical methods are being developed to quantitate unique DNA base damages in x-irradiated DNA. The approach is to chemically synthesize modified nucleotides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits. Several thymine ring saturation or fragmentation products have been chosen as models for pyrimidine radiolysis products. These include thymine glycol, dihydrothymine and urea. The characterized antibodies are used to quantitate DNA damage x-irradiated in vitro and to immunochemically characterize the antibody to dihydrothymine. 12 refs., 1 fig., 1 tab.

• Research Organization: New York Medical Coll., Valhalla (USA). Dept. of Microbiology and Immunology
• DOE Contract Number: FG02-87ER60510; AC02-80EV10417
• OSTI ID: 6122666
• Report Number(s): DOE/ER/60510-T1; ON: DE87014803
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DNA
DNA ADDUCTS
DNA REPAIR
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOASSAY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOLYSIS
RECOVERY
REPAIR
THYMINE
URACILS