NcoI RFLP at the creatine kinase-muscle type gene locus (CKMM, chromosome 19)

Journal Article · Mon Sep 12 04:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8743· OSTI ID:6949130

Coerwinkel-Driessen, M; Schepens, J; van Zandvoort, P; van Oost, B; Mariman, E; Wieringa, B ^[1]

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1. Univ. Nijmegen (Netherlands)

A 3.2 kbp human genomic DNA fragment (BamHI-Sau3A) of the 3{prime} untranslated and 3{prime} flanking region of the CKMM gene was isolated and subcloned into the BamHI site of vector pSP64. The CKMM 3{prime}-probe identifies a 2-allele polymorphism with bands at 2.3 and 1.0 kbp (allele A) and 3.3 kbp (allele B). In addition a weak constant 4.2 kbp band is observed. This probe also detects a 2-allele TaqI RFLP reported previously, as either a 4.3 kbp (A) or a 4.2 kbp (B) band. The CKMM locus previously has been assigned to 19q13.2-q13.3. By Southern blot analysis of human-rodent somatic cell hybrids containing unique subregional fragments of chromosome 19 of man the authors have assigned the gene to 19q13.2. Co-dominant segregation was observed in 8 families with 3 generations.

• OSTI ID: 6949130
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CHROMOSOMES
CREATINE
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 19
HUMAN POPULATIONS
HYBRIDIZATION
MAPPING
MUSCLES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
POPULATIONS
RECOMBINANT DNA
RFLPS
STRUCTURAL CHEMICAL ANALYSIS