NcoI and TaqI RFLPs for human M creatine kinase (CKM)

Journal Article · Mon Sep 12 04:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8744· OSTI ID:6810704

Perryman, M B; Hejtmancik, J F; Ashizawa, Tetsuo; Armstrong, R; Lin, Sunchiang; Roberts, R; Epstein, H F ^[1]

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1. Baylor College of Medicine, Houston, TX (USA)

Probe pHMCKUT contains a 135 bp cDNA fragment inserted into pGEM 3. The probe corresponds to nucleotides 1,201 to 1,336 located in the 3{prime} untranslated region of human M creatine kinase. The probe is specific for human M creatine kinase and does not hybridize to human B cretine kinase sequences. NcoI identifies a two allele polymorphism of a band at either 2.5 kb or 3.6 kb. TaqI identifies a two allele polymorphism at either 3.8 kb or 4.5 kb. Human M creatine has been localized to chromosome 19q. Autosomal co-dominant inheritance was shown in six informative Caucasian families.

• OSTI ID: 6810704
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CHROMOSOMES
CREATINE
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
ENZYMES
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 19
HUMAN POPULATIONS
HYBRIDIZATION
MAPPING
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POPULATIONS
RECOMBINANT DNA
RFLPS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES