A deletion/insertion polymorphism in the human BCR gene on chromosome 22

Journal Article · Mon Sep 12 00:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8741· OSTI ID:7101152

Rubin, C M; Hooberman, A L; Dickler, M N; Miller, B A; Westbrook, C A ^[1]; Blazar, B R ^[2]

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1. Univ. of Chicago, IL (USA)
2. Univ. of Minnesota, Minneapolis (USA)

A 2.3 kb single-copy genomic EcoRI-BamHI fragment was isolated from the putative first intron of the human BCR gene and subcloned into the Bluescribe vector pBS M13+ (pA-EB2.3). Multiple enzymes resolve a two-allele deletion/insertion type RFLP. A 1 kb difference between the alleles is seen in BamHI, Bg1II and HindIII digests. The deleted/inserted material includes EcoRI and TaqI sites; thus, using these enzymes the fragment lengths corresponding to the alleles do not differ by 1 kb. The BCR gene is within chromosome band 22q11. A family was studied in which the parents represented both homozygotes. The child was heterozygous as expected.

• OSTI ID: 7101152
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
ELECTROPHORESIS
GENES
GENETIC MAPPING
HEMIC DISEASES
HETEROCHROMOSOMES
HUMAN POPULATIONS
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
LEUKEMIA
MAPPING
NEOPLASMS
NUCLEIC ACIDS
ONCOGENES
ORGANIC COMPOUNDS
POPULATIONS
RECOMBINANT DNA
RFLPS
STRUCTURAL CHEMICAL ANALYSIS