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Haplotypes of the human immunoglobulin lambda IGLV7SI and IGLVISI genes defined by restriction-site polymorphism and insertion/deletion of a 6-kb DNA fragment

Journal Article · · American Journal of Human Genetics; (United States)
OSTI ID:5852585

Haplotypes were defined in the human immunoglobulin lambda locus by using three probes - V[lambda]VII, V[lambda]A, and V[lambda]I - hybridized to BamHI, KpnI, EcoRI, and HindIII digests. Four Kpnl alleles were described. Two of them, 13 kb and 16 kb, detected with both the V[lambda]VII and V[lambda]A probes, were correlated with 4.6-kb and 10.5-kb KpnI fragments, respectively, which hybridize to the V[lambda]I probe. The two others (17 kb and 24 kb) were detected with the three probes V[lambda]VII, V[lambda]A, and V[lambda]I. Moreover, the authors show that two of those haplotypes may reflect an insertion of 6 kb between V[lambda]A and V[lambda]1S1. Familial studies were performed to demonstrate the Mendelian inheritance. These results demonstrate the absence of association between the C[lambda] alleles and V[lambda] haplotypes. 29 refs., 5 figs., 2 tabs.

OSTI ID:
5852585
Journal Information:
American Journal of Human Genetics; (United States), Journal Name: American Journal of Human Genetics; (United States) Vol. 53:2; ISSN AJHGAG; ISSN 0002-9297
Country of Publication:
United States
Language:
English

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