Genetics of formamidase-5 (brain formamidase) in the mouse: localization of the structural gene on chromosome 14

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Biochem. Genet.; (United States)

DOI:https://doi.org/10.1007/BF00498880· OSTI ID:6945957

Cumming, R B ^[1]; Walton, M F; Fuscoe, J C; Taylor, B A; Womack, J E; Gaertner, F H

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1. Oak Ridge National Lab., TN

A single formamidase, which is different from the formamidases found in other tissues, occurs in the brains of mice. This enzyme is here called formamidase-5 and the gene symbol is designated For-5. Two alleles are recognized on the basis of their differential heat sensitivity: FOR-5/sup b/ is relatively heat stable and is present in strain C57BL/6J, while For-5/sup d/ is relatively heat sensitive and is present in strain DBA/2J. The heat sensitivity of formamidase-5 in 44 other inbred strains and substrains was tested and found to resemble that of C57BL/6J or DNA/2J. Thiry-six recombinant inbred strains derived from progenitors that differed at For-5 were studied to test for single-gene inheritance and linkage with other loci. Complete concordance was found with the esterase-10 locus (Es-10), indicating close linkage. The 99% upper confidence limit of the distance between For-5 and Es-10 is 3.7 centimorgans (cM). Es-10 is located on chromosome 14 about 19 cM from the centromere. An independent demonstration of linkage of For-5 with Es-10 and another chromosome 14 marker, hairless (hr), is provided by the finding that the HRS/J strain, which has been sibmated for 60 generations with forced heterozygosity at the hr locus, is cosegregating at For-5 and Es-10. A survey of 32 inbred strains and substrains revealed that the For-5/sup d/ allele is associated with th Es-10/sup b/ allele, and that the For-5/sup b/ allele is associated with Es-10/sup a/ and Es-10/sup c/. Formamidase-5 segregates as expected in the F/sub 2/ generation of crosses between strains bearing For-5/sup b/ and For-5/sup d/ alleles. It is possible that this unique formamidase of the brain is involved in the metabolism of a neurotransmitter substance.

• OSTI ID: 6945957
• Journal Information: Biochem. Genet.; (United States), Journal Name: Biochem. Genet.; (United States) Vol. 17:5/6; ISSN BIGEB
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
ENZYMES
GENES
GENETIC MAPPING
GENETICS
MAMMALS
MAPPING
MICE
NERVOUS SYSTEM
ORGANS
RODENTS
VERTEBRATES