Genetic variation of an intestinal leucine arylaminopeptidas (Lap-1) in the mouse and its location on chromosome 9

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Biochem. Genet.; (United States)

DOI:https://doi.org/10.1007/BF00484910· OSTI ID:7324029

Womack, J E; Lynes, M A; Taylor, B A

Electrophoretic variation for an intestinal enzyme that cleaves L-leucyl-..beta..-naphthylamide has been discovered among inbred mouse strains. Several strains including related strains C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, and C58/J demonstrate an electrophoretic band of this enzyme that is absent in other strains and stocks thus far observed. The enzyme is tentatively being called leucine arylaminopeptidase (LAP) and the variant genetic locus Lap-1. The presence of the band is determined by an allele designated Lap-1/sup a/. Homozygotes for the alternate allele, Lap-1/sup b/, are without the band and heterozygotes are, under our electrophoretic conditions, indistinguishable from Lap-1/sup a/ homozygotes. Data from recombinant inbred lines and a B6D2F/sub 1/ x DBA/2J backcross established linkage of Lap-1 to dilute (d) and supernatant malic enzyme (Mod-1) on chromosome 9 in the following order: Lap-1-d-Mod-1. The Lap-1 to d map distance was estimated to be 21.3 +- 4.6 cM from backcross data and 8.1 +- 4.8 cM from recombinant inbred lines.

• Research Organization: Jackson Lab., Bar Harbor, ME
• OSTI ID: 7324029
• Journal Information: Biochem. Genet.; (United States), Journal Name: Biochem. Genet.; (United States) Vol. 13:9/10; ISSN BIGEB
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOLOGICAL VARIABILITY
BIOLOGY
CARBOXYLIC ACIDS
CHROMOSOMES
DATA ANALYSIS
ELECTROPHORESIS
ENZYMES
GENETIC MAPPING
GENETIC VARIABILITY
GENETICS
HYDROLASES
LEUCINE
MAMMALS
MICE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
RODENTS
VERTEBRATES