Radioimmunoassay of progesterone in unextracted serum. [/sup 3/H]
A rapid, precise radioimmunoassay for progesterone in 25 ..mu..L of unextracted serum is described. Progesterone is released from its binding protein by adding an optimal amount of cortisol, which binds to the same protein (cortisol binding globulin) as progesterone. The amount of cortisol required does not cross react with the specific progesterone antibody used. This approach considerably shortens assay time and removes a tedious and imprecise stage in the conventional assay of serum progesterone. Results correlated well (r = 0.97) with a method involving organic solvent extraction of progesterone from serum. During the two years we have used this mehod in a busy diagnostic endocrine laboratory, the between-assay precision (CV) for low-, medium-, and high-concentration quality control sera was 12, 7, and 9%, respectively. Data from participation in an independent external quality-control program verified the adequacies of the method.
- OSTI ID:
- 6945799
- Journal Information:
- Clin. Chem.; (United States), Vol. 26:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PROGESTERONE
RADIOIMMUNOASSAY
ANTIBODIES
BLOOD SERUM
IMMUNE SERUMS
PATIENTS
QUALITY CONTROL
TRITIUM COMPOUNDS
CONTROL
HORMONES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques