Simultaneous measurement of triiodothyronine and thyroxine in unextracted serum samples using a double-tracer radioimmunoassay method. [/sup 125/I, /sup 131/I]

Journal Article · Tue Mar 01 04:00:00 EST 1977 · J. Nucl. Med.; (United States)

OSTI ID:7223074

Brown, M L; Hannah, T; Kopp, D T; Stephens, G; Carretta, R F

A method is described for the simultaneous measurement of triiodothyronine (T/sub 3/) and thyroxine (T/sub 4/) in 0.04 ml of unextracted serum. Antibodies were prepared by immunization of rabbits with T/sub 3/ and T/sub 4/ conjugated with human serum albumin. Bound and free labeled hormones were separated by the double-antibody technique, and 8-anilino-1-naphthalene sulfonic acid was used to inhibit binding of the two hormones to thyroxine-binding globulin. The validity of the assay using /sup 125/I-T/sub 3/ and /sup 131/I-T/sub 4/ is shown by the excellent recovery of T/sub 3/ and T/sub 4/ added to serum and by the finding that curves obtained by assaying various dilutions of a hyperthyroid serum run parallel to the standard curves. In all clinical states, serum T/sub 3/ and T/sub 4/ values obtained using the double-tracer radioimmunoassay method were in excellent agreement with those obtained by single-tracer RIA techniques. The combined T/sub 3/-T/sub 4/ method appears to be accurate, sensitive, and specific, thus making the assay highly desirable as a technical time-saver.

• Research Organization: Lackland Air Force Base, TX
• OSTI ID: 7223074
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 18:3; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
AMINO ACIDS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD SERUM
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
TRIIODOTHYRONINE